gata4  (Bioss)

Journal: Current Issues in Molecular Biology

Article Title: Sodium Butyrate Ameliorated Bile Acid Metabolism in Diabetes Mellitus by PI3K/AKT Signaling Pathway via the Gut–Liver Axis

doi: 10.3390/cimb47090732

Figure Lengend Snippet: Monocellular barrier function analysis in CaCo 2 cells. Immunofluorescence assay for ( A ) ZO-1, ( B ) VE, ( C ) Occludin, ( D ) Claudin-5, ( G ) FXR, ( H ) PTPN6, and ( I ) GATA4 under a laser scanning confocal microscope, and fluorescence intensity was determined relative to each other. ( E ) The TEER of CaCo 2 cells was increased from Day 0 to Day 21; ( F ) Insulin + LPS increased transendothelial albumin passage across the monocellular barrier; ** p < 0.01, *** p < 0.001, vs. control (Day 0 or 0H). Intracellular content of TBA in ( J ) CaCo 2 was determined by kit; *** p < 0.001, n = 3.

Article Snippet: Primary antibodies against GPR43 (bs-13536R), PTPN6 (bs-4158R), GATA4 (bs-1778R), GLUT2 (bs-0351R), Insulin Receptor (bs-0681R), PI3K (bs-10657R), and p-PI3K (bs-6417R) were obtained from Bioss (Peking, China).

Techniques: Immunofluorescence, Microscopy, Fluorescence, Control

Journal: Current Issues in Molecular Biology

Article Title: Sodium Butyrate Ameliorated Bile Acid Metabolism in Diabetes Mellitus by PI3K/AKT Signaling Pathway via the Gut–Liver Axis

doi: 10.3390/cimb47090732

Figure Lengend Snippet: Effect of NaB on bile acid metabolism-related proteins in HepG 2 cells. Sodium butyrate (NaB) increased FXR, TGR5, PTPN6, GATA4, and CYP7A1 expression. Immunofluorescence assay for ( A ) FXR, ( B ) TGR5, ( C ) PTPN6, ( D ) GATA4, and ( E ) CYP7A1 under a laser scanning confocal microscope, and fluorescence intensity was determined relative to each other. ( F ) The above proteins were analyzed using Western blotting. The PI3K/AKT signaling pathway participated in the function of NaB. ( G ) WB detection of PI3K/AKT/GSK3 signaling proteins, including the ratio of p-PI3K to total PI3K, ratio of p-AKT to total AKT, and ratio of p-GSK3α to total GSK3α; ( E ) ratio of p-GSK3β to total GSK3β. * p < 0.05, ** p < 0.01, *** p < 0.001, vs. Ins + LPS, n = 3. shows the native image of the Western blot of .

Article Snippet: Primary antibodies against GPR43 (bs-13536R), PTPN6 (bs-4158R), GATA4 (bs-1778R), GLUT2 (bs-0351R), Insulin Receptor (bs-0681R), PI3K (bs-10657R), and p-PI3K (bs-6417R) were obtained from Bioss (Peking, China).

Techniques: Expressing, Immunofluorescence, Microscopy, Fluorescence, Western Blot

Journal: F1000Research

Article Title: Reversine enhances the conversion of dedifferentiated fat cells into mature cardiomyocytes

doi: 10.12688/f1000research.122788.3

Figure Lengend Snippet: Median value and Kruskal-Wallis test for GATA4 and cTnT based on the concentration of reversine.

Article Snippet: For double staining, the slides were incubated with Anti-Rabbit GATA4 Polyclonal Antibody (1:100 dilution; Thermo Fisher Scientific, Inc., Waltham, MA, USA: Catalog # BS-1778R) and Mouse IgG Monoclonal Antibody against Cardiac troponin T Recombinant (1:300 dilution; LSBio (LifeSpan) Cat# LS-C89784-200, RRID:AB_1936961) at 4°C overnight and then washed three times with PBS.

Techniques: Concentration Assay

Journal: F1000Research

Article Title: Reversine enhances the conversion of dedifferentiated fat cells into mature cardiomyocytes

doi: 10.12688/f1000research.122788.3

Figure Lengend Snippet: GATA4, Transcription factor GATA-4; REV, reversine.

Article Snippet: For double staining, the slides were incubated with Anti-Rabbit GATA4 Polyclonal Antibody (1:100 dilution; Thermo Fisher Scientific, Inc., Waltham, MA, USA: Catalog # BS-1778R) and Mouse IgG Monoclonal Antibody against Cardiac troponin T Recombinant (1:300 dilution; LSBio (LifeSpan) Cat# LS-C89784-200, RRID:AB_1936961) at 4°C overnight and then washed three times with PBS.

Techniques:

Journal: F1000Research

Article Title: Reversine enhances the conversion of dedifferentiated fat cells into mature cardiomyocytes

doi: 10.12688/f1000research.122788.3

Figure Lengend Snippet: Oct4, Octamer-binding transcription factor 4; Flk-1, Fetal liver kinase 1; GATA4, Transcription factor GATA-4; cTnT, Cardiac troponin T.

Article Snippet: For double staining, the slides were incubated with Anti-Rabbit GATA4 Polyclonal Antibody (1:100 dilution; Thermo Fisher Scientific, Inc., Waltham, MA, USA: Catalog # BS-1778R) and Mouse IgG Monoclonal Antibody against Cardiac troponin T Recombinant (1:300 dilution; LSBio (LifeSpan) Cat# LS-C89784-200, RRID:AB_1936961) at 4°C overnight and then washed three times with PBS.

Techniques: Binding Assay